Plasmid_Backbone

Part:BBa_K4435312:Design

Designed by: Elizabeth Bilsland   Group: iGEM22_Unicamp_Brazil   (2022-10-08)


level 2 JUMP plasmid, pBBR1 ori, CmR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2852
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2852
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2852
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2852
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2867
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2874
    Illegal BsaI.rc site found at 10


Design Notes

Validated by loss of resistance to spectinomycin and gain of resistance to chloramphenicol


Source

Amplification of BBa_J428367 with JUMP_mF and JUMP_mF; amplification of antibiotic resistance marker CmR from BBa_J428357; Golden gate cloning with SapI

References